Automated measurement of serum ferroxidase activity

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Automated measurement of serum ferroxidase activity.

A method is described for automated measurement of serum ceruloplasmin ferroxidase activity. In this method, Fe2+ ions are used as the substrate. In addition, a new calibration system without ceruloplasmin is also presented. Optimum assay reaction conditions were determined. Maximal catalytic activity was obtained at 0.45 mol/L acetate buffer, pH 5.8. The reagents and calibrator are stable for ...

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Automated determination of serum alpha1-antitrypsin by antitryptic activity measurement.

BACKGROUND Alpha1-Antitrypsin (A1AT) deficiency is currently detectable by protein immunoassay, phenotyping, and genotyping of the S and Z mutations, but no fully automated method for standard biochemical analyzers is yet available. Here, we present a method that measures the antitryptic activity in serum. This method is rapid, automated, and allows the easy evaluation of a large cohort of pati...

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Automated Determination of Serum 1-Antitrypsin by Antitryptic Activity Measurement

BACKGROUND: 1-Antitrypsin (A1AT) deficiency is currently detectable by protein immunoassay, phenotyping, and genotyping of the S and Z mutations, but no fully automated method for standard biochemical analyzers is yet available. Here, we present a method that measures the antitryptic activity in serum. This method is rapid, automated, and allows the easy evaluation of a large cohort of patients.

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Ferroxidase activity of rat ceruloplasmin.

WILLIAMS, ID. M., G. K. LEE, AND G. E CARTWRIGIIT. Fb-roxidasc nctivity of rat ccruloplasmin. Am. J. Physiol. 227(5) : 1094-1097. 1974.-The ferroxidase activity of rat ceruloplasmin was studied under various assay conditions. The ferroxidase activity was optimal at pH 6.0-6.2 in acetate buffer in the absence of ascorbate, and under these conditions the activity was about 1/3rd that of human cer...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 1998

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/44.11.2313